Herpes + MBV -coley recipe -- Preparation of bacterial toxins

Preparation of bacterial toxins.

H. F. HAVAS,M. E. GROESBECK, AND A. J. DONNELLY

(The Institute for Cancer Research and The Lankenau Hospital Research Institute, Philadelphia, Pa.)

One-tenth of a milliliter of a
24-hour broth culture of Streptococcus pyogenes or Serratia
marcescenswas inoculated into 50ml. of Neopeptone broth (10
gm. Neopeptone, 5 gm. NaCl, 3 gm. beef extract per liter of
double-distilled water), and incubated at 37°and 25°C., re-
spectively. Depending on the type ofpreparation, the Strepto-
cocci were grown 4 weeks, 2 weeks, or 4 days; Serratia, 2
weeks, 1week, or 2days. Either the cultures were grown sepa-
rately and mixed before heat sterilization, or Serratia marces-
censis inoculated into the growing streptococcus culture at a
specified time depending on the type of preparation ("s" indi
cates grown separately and "f" together, see tables). The cul
tures were heat-sterilized at 68°C. for 90minutes and routinely
checked for sterility in Thioglycollate broth (Difco). The stor
age of all preparations in the cold room at 4°C. caused no
changed in potency when tested up to 1J years later.
Tumor assay with Sarcoma37.â€"Ascitesfluid containing ap
proximately 3.5 million cells (0.25 ml. of fluid diluted 1:2 with
Ringer) was inoculated subcutaneously between the shoulders
of 7-8-week-old Swiss mice. After 7 days, mice with tumors
ranging from 1.5 to 2.5 sq. cm. at the base, as measured by a
series ofcalibrated rings, were utilized in the experiments. Mice
were tested in groups of sixteen or multiples thereof. Survival
time, tumor size, and percentage of complete regressions were
all considered as indices ofefficacy. However, the final criterion
of the effectiveness ofthe toxins was the complete regression of
the tumor at the termination of the experiment and not a
partial reduction in tumor size. For the determination of re
gression and survival data, experiments were terminated 60-80
days after the tumor implantation, when the last tumor-bearer
had died and only mice free of detectable tumor remained.
In the toxicity studies, 7-8 week-old-male Swiss mice were
given injections routinely of 0.05 or 1.0 ml. of toxins. Higher
dosages ofpreparations ofboth high and low toxicity were also
given to obtain LD100 values.

http://cancerres.aacrjournals.org/content/18/2/141.full.pdf


Moullin's  conclusions  as to the value of toxin therapy
are:  (a)  That  a  considerable  numbe'r  of  hopelessly
inoperable  sarcomas,  many  of  which  were  recurrent,
have  completely  regressed  under  this  treatment,  and
that  there  is  no  other  of  which  this  can  be  said.
(b)  That  the  disappearance  of  sarcoma  is  not due
to  inflammation,  but  to  an  intensely  rapid  form  of
fatty  degeneration  comparable  only  to  that  which
affects  the  hepatic  cells  in  acute  yellow  atrophy  of  the
liver. 

(j) That  disappearance 
of  the  growths  is  not  the  result  of  high  temperature
alone,  since  fever  from  other  causes  is  not  followed
by  this  result  (51).


HERPES

"Cephalalgia  sometimes  occurred,  as well as nausea
and  slight  vertigo.  Herpes  labialis,  sweating  of  the
palms  of  the  hands,  and  fugacious  eruptions  were
observed  in  some  cases in  which  the  fever  was  well
marked.


THE TREATMENT OF EXPERIMENTAL TUMORS WITH BACTERIAL TOXINS.
S. P. BEEBE; MARTHA TRACY
J Am Med Assoc. 1907;XLIX(18):1493-1498.


THE TREATMENT OF EXPERIMENTAL TUMORS WITH BACTERIAL TOXINS.

S. P. BEEBE, M.D.; MARTHA TRACY, M.D.

JAMA. 1907;XLIX(18):1493-1498.

   
The use of injections of the combined sterilized cultures of Streptococcus and the Bacillus prodigiosus in the treatment of inoperable sarcoma has acquired considerable prominence since its introduction by W. B. Coley of New York some fifteen years ago.

It is well known that intercurrent attacks of erysipelas have been observed in a number of cases to exert a restraining and even curative influence on the course of malignant tumors, and investigators have endeavored by inoculation with streptococcus cultures to bring about artificially equally beneficent results. Roger of Paris, in experimenting on rabbits, believed that by an admixture of the B. prodigiosus with his streptococcus he could enhance the virulence of the latter cultures, and Coley1 applied this idea to the treatment of sarcoma, beginning in 1892 a systematic clinical study of the therapeutic effect of such mixed toxins. The striking results attained in an increasing number of cases . . . [Full Text PDF of this Article]


Author Affiliations
    NEW YORK CITY.
From the Huntington Fund for Cancer Research of the General Memorial Hospital. The laboratory work forming the basis of the report was done at the Loomis Laboratory, Cornell University, Medical College, New York.


Footnotes
    Read in the Section on Pathology and Physiology of the American Medical Association, at the Fifty-eighth Annual Session held at Atlantic City, June, 1907.

http://jama.ama-assn.org/cgi/content/summary/XLIX/18/1493
http://jama.ama-assn.org/cgi/reprint/XLIX/18/1493  << buy this!  Recipe!

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